Barrier systems such as isolators or RABS play a fundamental role in the protection of people and products in pharmaceutical production. Only tenths of a millimeter thick gloves are the most crucial interface in these hermetically sealed units. To ensure that barrier systems are sterile, and processes remain safe, a thorough and regular inspection of the glove integrity is vital. The regulatory requirements for gloves are strict. However, they also leave plenty of choice for suitable test methods. Many facility operators face the question as to which method is suited best for which system.